Fabricio Medina-Bolivar - Associate Professor

Phone: 870-680-4319 (Office)
Address: P.O. Box 639 I State University AR 72467
Email: fmedinabolivar@astate.edu


ASU: Biology and Arkansas Biosciences Institute

Cluster Identification:
- Comparative Metabolomics, Genomics and Metabolic Engineering Cluster
- Plant Interactions with Other Organisms Cluster

Research Areas/Expertise:
- Antioxidants
- Hairy Root Cultures
- Metabolic Engineering
- Plant Secondary Metabolism
- Phytochemicals & Human Health
- Phytochemical Analysis: HPTLC, HPLC, LC-MS
- Plant Tissue Culture
- Transgenic Plants

Research Summary | Selected Publications | Lab Members | Key Collaborators | Research Projects | Links

Research Summary:

The Medina-Bolivar laboratory focuses on elucidating the mechanisms that control the biosynthesis of selected plant natural products of importance to human health. We utilize hairy root cultures as sustainable bioproduction systems and elicitation and metabolic engineering strategies to increase the levels of these biologically active phytochemicals.  Our areas of study center on the biosynthesis and bioproduction of stilbenoids, flavonoids and alkaloids.

Selected Publications:

Brents LK, Medina-Bolivar F (*), Seely KA, Nair V, Bratton SM, Gallus-Zawada A, Ñopo-Olazabal L, Patel RY, Liu H, Doerksen RJ, Prather P, Radominska-Pandya A. 2012. Natural prenylated resveratrol analogs, arachidin-1 and -3, demonstrate an improved glucuronidation profile and have affinity for cannabinoid receptors. Xenobiotica. 42:139-156. [*First two authors share first authorship]

Joshee N, Tascan A, Medina-Bolivar F, Parajuli P, Rimando AM, Shannon DA, Adelberg JW. 2012. Scutellaria: Biotechnology, Phytochemistry and its Potential as a Commercial Medicinal Crop. Biotechnology for Medicinal Plants: Micropropagation and Improvement, Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer-Verlag, Heidelberg Germany (in press).

Nopo L, Woffenden B, Reed D, Buswell S, Zhang C, Medina-Bolivar F. 2012. Super-promoter: TEV, a powerful gene expression system for tobacco hairy roots. (In: Recombinant Gene Expression: Reviews and Protocols, Third Edition, A. Lorence (Ed.), Springer). Methods in Molecular Biology. 824:501-526.

Condori J, Nopo-Olazabal C, Medrano G, Medina-Bolivar F. 2011. Selection of reference genes for qPCR in hairy roots cultures of peanut. BMC Research Notes. 4:392 (20 pages)

Condori J, Sivakumar S, Hubstenberger J, Dolan MC, Sobolev VS, Medina-Bolivar F. 2010. Induced biosynthesis of resveratrol and the prenylated stilbenoids arachidin-1 and arachidin-3 in hairy root cultures of peanut: Effects of culture medium and growth stage. Plant Physiology and Biochemistry. 48:310-318.

Abbott J, Medina-Bolivar F, Martin E, Engelberth AS, Villagarcia H, Clausen EC, Carrier DC. 2010. Purification of resveratrol, arachidin-1 and arachidin-3 from hairy root culture of peanut (Arachis hypogaea) and determination of their antioxidant activity and cytotoxicity. Biotechnology Progress. 26:1344-1351.

Medina-Bolivar F, Dolan M, Bennett S, Condori J, Hubstenberger J. 2010. Production of stilbenes in hairy roots. US Patent N. 7666677.

Condori J, Medrano G, Sivakumar G, Nair V, Cramer C, Medina-Bolivar F. 2009. Functional characterization of a stilbene synthase gene using a transient expression in planta. Plant Cell Reports 28:589-599.

Medina-Bolivar F, Condori J, Rimando A, Hubstenberger J, Shelton K, Bennett S, Dolan M. 2007. Production and secretion of resveratrol in hairy root cultures of peanut. Phytochemistry. 68:1992-2003.

Lab Members:


Fabricio Medina-Bolivar, PhD,
Associate Professor
Arkansas Biosciences Institute and Department of Biological Sciences
fmedinaboliver@astate.edu ; 870-680-4319

Luis Nopo-Olazabal, PhD
Research Associate; Adjunct Faculty
Department of Biological Sciences
lnopo@astate.edu ; 870-680-4320


Cesar Nopo-Olazabal
PhD Student
Molecular Biosciences Program
cesar.nopoolazabal@astate.edu ; 870-680-4320


Tianhong Yang
M.S. Student
tianhong.yang@smail.astate.edu ; 870-680-4320


Zach Marsh
Honors - Undergraduate Student
zach.marsh@smail.astate.edu ; 870-680-4320


Linda Ogutu
Honors - Undergraduate Student
linda.ogutu@smail.astate.edu ; 870-680-4320


Yeun-Kyung Chang
Research Assistant
ykchang@astate.edu ; 870-680-4320


Ananddeep Patel
Research Assistant
ananddeep.patel@smail.astate.edu ; 870-680-4320


For detailed information of former laboratory members please visit: http://www.clt.astate.edu/fmedinabolivar/


Key Collaborators

Dr. Anna Radominska-Pandya, Professor, University of Arkansas for Medical Sciences, Little Rock
Dr. Malathi Srivatsan, Associate Professor, Arkansas State University, Jonesboro, AR
Dr. Nirmal Joshee, Research Associate Professor, Fort Valley State University, Fort Valley, GA
Dr. Agnes Rimando, Research Chemist, USDA-ARS Natural Products Utilization Research Unit, Oxford, MS
Dr. Paul Prather, Associate Professor, University of Arkansas for Medical Sciences, Little Rock
Dr Robert Doerksen, Associate Professor, University of Mississippi. Oxford, MS
Dr. Anastasios Karydis, Assistant Professor, University of Tennessee for Health Sciences, Memphis, TN

Research Projects

-Bioproduction of resveratrol and related stilbenoids in hairy root cultures of peanut and muscadine grape

-Elucidation of stilbenoid biosynthesis in peanut

-Biological activity of natural prenylated resveratrol analogs

-Metabolic engineering of flavonoid biosynthesis in Scutellaria lateriflora (American skullcap) by expression of selected transcription factors in hairy roots

-Bioproduction of anticancer alkaloids in tissue cultures of monocot species


Medina-Bolivar laboratory at Arkansas State University (hosted by the Medina-Bolivar laboratory): http://www.clt.astate.edu/fmedinabolivar/


Dr. Medina-Bolivar was the Program Chair and Host of the 3rd Annual Conference of American Council for Medicinally Active Plants (ACMAP). For a highlight of the conference check the following video: http://www.youtube.com/watch?v=9VEwO6fYCgQ&feature=plcp

American Council for Medicinally Active Plants: http://www.acmap.org

Medina-Bolivar's P3 YouTube Video: http://youtu.be/LNs5L_q6e84

P3 Stilbenoid Symphony YouTube Video: http://www.youtube.com/watch?v=sm6_p6DBJRg