ASU: Biology and Arkansas Biosciences Institute
- Comparative Metabolomics, Genomics and Metabolic Engineering Cluster
- Plant Interactions with Other Organisms Cluster
- Hairy Root Cultures
- Metabolic Engineering
- Plant Secondary Metabolism
- Phytochemicals & Human Health
- Phytochemical Analysis: HPTLC, HPLC, LC-MS
- Plant Tissue Culture
- Transgenic Plants
The Medina-Bolivar laboratory focuses on elucidating the mechanisms that control the biosynthesis of selected plant natural products of importance to human health. We utilize hairy root cultures as sustainable bioproduction systems and elicitation and metabolic engineering strategies to increase the levels of these biologically active phytochemicals. Our areas of study center on the biosynthesis and bioproduction of stilbenoids, flavonoids and alkaloids.
Marsh Z, Yang T, Nopo-Olazabal L, Wu S, Ingle T, Joshee N, Medina-Bolivar F. 2014. Effect of light, methyl jasmonate and cyclodextrin on production of polyphenolic compounds in hairy root cultures of Scutellaria Iaterifora. Phytochemistry 107:50-60.
Zhai B, Clark J, Ling T, Connelly M, Medina-Bolivar F, Rivas F. 2014. Antimalarial evaluation of the chemical constituents of hairy root culture of Bixa orellana. Molecules. 19(1):756-766.
Nopo-Olazabal C, Nopo-Olazabal L, Medina-Bolivar F. 2014. Differential induction of antioxidant stilbenoids in hairy roots of Vitis rotundifolia treated with methyl jasmonate and hydrogen peroxide. Plant Physiology and Biochemistry. 74:50-69.
Nopo-Olazabal C, Hubstenberger J, Nopo-Olazabal L, Medina-Bolivar F. 2013. Antioxidant activity of selected stilbenoids and their bioproduction in hairy root cultures of muscadine grape (Vitis rotundifolia Michx.). Journal of Agricultureal and Food Chemistry. 61(48):11744-11758
Joshee N, Tascan A, Medina-Bolivar F, Parajuli P, Rimando AM, Shannon DA, Adelberg JW. 2013. Scutellaria: Biotechnology, Phytochemistry and its Potential as a Commercial Medicinal Crop. In: Biotechnology for Medicinal Plants: Micropropagation and Improvement, Eds. Suman Chandra, Hemant Lata and Ajit Varma, Springer-Verlag, Heidelberg Germany. pp. 69-99.
Prabhu P, Medina-BolivarF, Condori J, Sivakumar G, Srivatsan M. 2012. Neuroprotective effect of peanut hairy root extract against oxidative stress in PC12 derived neurons. Journal of Medicinally Active Plants. 1(4):125-133.
Brents LK, Medina-Bolivar F (*), Seely KA, Nair V, Bratton SM, Gallus-Zawada A, Nopo Olazabal L, Patel RY, Liu H, Doerksen RJ, Prather P, Radominska-Pandya A. 2012. Natural prenylated reseratrol analogs, arachidin-1 and -3, demonstrate an improved glucuronidation profile and have affinity for cannablnold receptors. Xenobiotica. 42:139-156. [*First two authors share first authorship]
Nopo L, Woffenden B, Reed D, Buswell S, Zhang C, Medina-Bolivar F. 2012. Super-promoter: TEV, a powerful gene expression system for tobacco hairy roots. (In: Recombinant Gene Expression: Reviews and Protocols, Third Edition, A. Lorence (Ed.), Springer). Methods in Molecular Biology. 824:501-526.
Condori J, Nopo-Olazabal C, Medrano G, Medina-Bolivar F. 2011. Selection of reference genes for qPCR in hairy roots cultures of peanut. BMC Research Notes. 4:392
Sivakumar G, Medina-Bolivar F, Lay J, Dolan MC, Condori J, Wright SM, Baque MDA, Lee E-J, Paek KY. 2011. Bioprocess and bioreactor: Next generation technology for production of potential plant-based antidiabetic and antioxidant molecules. Current Medicinal Chemistry. 18:79-90.
Abbott J, Medina-Bolivar F, Martin E, Engelberth AS, Villagarcia H, Clausen EC, Carrier DC. 2010. Purification of resveratrol, arachidin-1 and arachidin-3 from hairy root culture of peanut (Arachis hypogaea) and determination of their antioxidant activity and cytotoxicity. Biotechnology Progree. 26(5):1344-1351
Condori J, Sivakumar S, Hubstenberger J, Dolan MC, Sobolev VS, Medina-Bolivar F. 2010. Induced biosynthesis of reveratrol and the prenylated stillbenolds arachihinn-1 and archidin-3 in hairy root cultures of peanut: Effects of culture medium and growth stage. Plant Physology and Biochemstry. 48:310-318.
Condori J, Medrano G, Sivakumar G, Nair V, Cramer C, Medina-Bolivar F. 2009. Functional characterization of a stilbene synthase gene using a transient expression in planta. Plant Cell Reports 28:589-599.
Medina-Bolivar F, Condori J, Rimando A, Hubstenberger J, Shelton K, Bennett S, Dolan M. 2007. Production and secretion of resveratrol in hairy root cultures of peanut. Phytochemistry. 68:1992-2003.
|Fabricio Medina-Bolivar, Ph.D.
Associate Professor of Metabolic Engineering
Department of Biological Sciences
Arkansas Biosciences Institute
firstname.lastname@example.org; (870) 680-4319
|Luis Nopo-Olazabal, Ph.D.
|Tianhong Yang, M.S.
Molecular Biosciences Graduate Program
email@example.com; (870) 680-4320
firstname.lastname@example.org; (870) 680-4320
email@example.com; (870) 680-4320
firstname.lastname@example.org; (870) 680-4320
email@example.com; (870) 680-4320
Honors Undergraduate Student
Biology and Chemistry
firstname.lastname@example.org; (870) 680-4320
email@example.com; (870) 680-4320
|Christopher Aaron Tollett
Honors Undergraduate Student
Biology and Chemistry
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|Dr. Cesar Nopo-Olazabal
Graduated: Summer 2013 (Ph.D. Molecular Biosciences)
Now: Research Scientist; Altria, Richmond, Virginia
Graduated: Spring 2013 (Honors, Biology)
Now: Master's student, Public Health in Epidemiology; Emory University, Atlanta, Georgia
Graduated: Spring 2012 (M.S. Biotechnology)
Now: Ph.D. student, Veterinary Biomedical Sciences; Oklahoma State University, Stillwater, Oklahoma
Graduated: Fall 2011
Now: Master's student, Crop Biotechnology and Entrepreneurship; The University of Nottingham, England
|Anandeep Patel (M.S. Biotechnology)
Graduated: Spring 2013
Now: Research Technician; Texas Medical Center, Houston, TX
For detailed information of former laboratory members, please visit: http://www.clt.astate.edu/fmedinabolivar
Dr. Nirmal Joshee, Fort Valley State University, Fort Valley, Georgia
Dr. Keithanne Mockaitis, Associate Scientist, Indiana University, Bloomington, Indiana
Dr. Anna Radominska-Pandya, Professor, University of Arkansas for Medical Sciences, Little Rock
Dr. Agnes Rimando, USDA, Natural Products Utilization Research Unit, Oxford, Mississippi
Dr. Malathi Srivatsan, Profesor, Arkansas State University, Jonesboro, Arkansas
Dr. Brett Savary, Professor, Arkansas State University, Jonesboro, Arkansas
Dr. Victor Sobolev, USDA, National Peanut Research Laboratory, Dawson, Georgia
-Bioproduction of resveratrol and related stilbenoids in hairy root cultures of peanut and muscadine grape
-Elucidation of stilbenoid biosynthesis in peanut
-Biological activity of natural prenylated resveratrol analogs
-Metabolic engineering of flavonoid biosynthesis in Scutellaria lateriflora (American skullcap) by expression of selected transcription factors in hairy roots
-Bioproduction of anticancer alkaloids in tissue cultures of monocot species
Medina-Bolivar laboratory at Arkansas State University (hosted by the Medina-Bolivar laboratory): http://www.clt.astate.edu/fmedinabolivar/
Dr. Medina-Bolivar was the Program Chair and Host of the 3rd Annual Conference of American Council for Medicinally Active Plants (ACMAP). For a highlight of the conference check the following video: http://www.youtube.com/watch?v=9VEwO6fYCgQ&feature=plcp
American Council for Medicinally Active Plants: http://www.acmap.org
Medina-Bolivar's P3 YouTube Video: http://youtu.be/LNs5L_q6e84
P3 Stilbenoid Symphony YouTube Video: http://www.youtube.com/watch?v=sm6_p6DBJRg